詳細說明

Lolina A/S

Address: Sindalsvej 30 8240 Risskov Danmark 

                    Email: Info@lolina.dk

Product Specification

Product Description

Lolina? Nucleus Pulposus Cell Growth Supplement (NPCGS) is a sterile, concentrated (100X) solution which  contains  growth  factors,  hormones,  and  proteins  necessary  for  the  culture  of normal nucleus  pulposus  cells.  The  supplement  is  formulated  (quantitatively  and  qualitatively)  to provide  an  optimally  balanced  environment  that  selectively  supports  the  growth  of  normal nucleus pulposus cells in vitro.

Instructions for Use

For best results, use NPCGS in conjunction with 500 ml Human Nucleus Pulposus Cell Medium- b (HNPC-b) and 10 ml fetal bovine serum (FBS).

Thaw NPCGS at 37^oC. Gently tilt the tube several times to ensure complete mixing. Spray the medium bottle and supplement tube with 70% ethanol and wipe to remove excess liquid. In a sterile field, remove the caps without touching the interior threads with fingers. Add NPCGS to the basal medium and mix well. Since several components are light-labile, the medium should not be exposed to light for extended periods. We do not recommend warming medium in a 37^oC water bath prior to use. When stored in the dark at 4^oC, the reconstituted medium is stable for one month.

Caution: If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.

Note

NPCGS is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

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