詳細(xì)說(shuō)明

Lolina A/S

Address: Sindalsvej 30 8240 Risskov Danmark 

                    Email: Info@lolina.dk

Product Specification

Product Description

Lolina? Hepatocyte Growth Supplement (HGS) is a sterile, concentrated (100X) solution which contains growth factors, hormones, and proteins necessary for the culture of normal hepatocytes. The supplement is  formulated  (quantitatively and  qualitatively) to provide an optimally balanced environment that selectively supports the growth of normal hepatocytes in vitro.

Instructions for Use

For best results, use HGS in conjunction with 500 ml Hepatocyte Medium-b (HM-b) and 25 ml fetal bovine serum (FBS).

Thaw HGS at 37oC. Gently tilt the tube several times to ensure complete mixing. Spray the medium bottle and supplement tube with 70% ethanol and wipe to remove excess liquid. In a sterile field, remove the caps without touching the interior threads with fingers. Add HGS to the basal medium and mix well. Since several components are light-labile, the medium should not be exposed to light for extended periods. We do not recommend warming medium in a 37oC water bath prior to use. When stored in the dark at 4oC, the reconstituted medium is stable for one month.

Caution: If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.

Note

HGS is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

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