詳細說明

Lolina A/S

Address: Sindalsvej 30 8240 Risskov Danmark 

                    Email: Info@lolina.dk

Product Specification

Product Description

Lolina? stem-Prom? supplement kit 1 is a sterile lyophilized products which contains growth factors and proteins for significant enhancement of BM-MSC proliferation and viability. The kit is usually used as a in vitro BM-MSC culture supplement.

It has the following benefit:

Enhance cell attachment and expansion ability: NaC20130101 can promote cell attachment and expansion on the culture matrix and enhance the interaction between cells and the matrix.

Provide essential nutrients and a stable environment: NaC20130101 can carry essential nutrients, provide the components required for cell metabolism, and maintain the stability of the culture environment.

Improve cell survival and proliferation rate: NaC20130101 can significantly improve the survival and proliferation rate of MSCs, especially under low serum or serum-free culture conditions.

Maintain stemness and multipotency: NaC20130101 helps maintain the stemness and multipotency of MSCs, prevent premature cell differentiation, and maintain cell proliferation ability.

Improve cell viability: NaC20130101 can improve the vitality of MSCs, enhance cell survival rate and anti-apoptosis ability.

Components

Instructions for Use

1. Stock solution Preparation.

One kit is for 50ml cell culture medium.

The compounds are offered as powder in tubes. Please centrifuge before opening the cap to ensure the accuracy of the dosage.

Please carry out dissolution and packaging operations on a clean bench.

Spray the medium bottle and supplement tube with 70% ethanol and wipe to remove excess liquid. In a sterile field, remove the caps without touching the interior  threads  with  fingers. 

Reconstitute the protein in sterile 1 × PBS pH 7.4. Aliquot into appropriate volumes of storage solution.

When stored at -20℃, the stock solution is stable for 6 month. When stored at 4 ℃, the stock solution is stable for 1 week.

2. Reference Protocol

Step 1: Culture medium Preparation

a.  Thaw stock solutions of Lolina? Human BM-MSC stem-Prom? supplement kit 1.

b.  Dilute the stock solutions into regular culture medium to get complete culture medium.

Step 2: Cell culture

c.   Rapidly thaw frozen BM-MSC vials in a 37°C water bath.

d.  Transfer cells to a sterile conical tube with pre-warmed medium.

e.   Centrifuge at 300g for 5 minutes to pellet cells.

f.   Resuspend the cell pellet in complete culture medium plate into culture dishes/flasks.

g.   Change the medium every 2-3 days.

Note

Cell Density: Avoid over-confluence to maintain MSC multipotency.

Medium Preparation: Use freshly prepared culture medium to ensure that it does not contain contaminants and degradation products.

Contamination Control: Use aseptic techniques to prevent contamination.

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