詳細(xì)說明

Lolina A/S

Address: Sindalsvej 30 8240 Risskov Danmark 

                    Email: Info@lolina.dk

Product Specification

Product Description

Lolina? stem-Prom? supplement kit 2 is a sterile products which focus on sustaining high cell expression and proliferation. The kit is usually used as a UC-MSC in vitro culture supplement.

It has the following benefit:

Protect cells from stress and damage: rHSA has antioxidant and anti-stress effects, which can reduce oxidative stress damage to cells during culture. Meanwhile SafeGluta? is stable in the culture environment. Unlike L-Glutamine, it can provide cells with a stable supply of nutrients without spontaneously degrading and producing ammonia.

Provide essential nutrients and a stable environment: NaC20120115 can carry essential nutrients, provide the components required for cell metabolism, and maintain the stability of the culture environment.

Improve cell survival and proliferation rate: Adding NaC20120115 can significantly improve the survival and proliferation rate of MSCs, especially under low serum or serum-free culture conditions.

Enhance cell attachment and expansion ability: NaC20120115 can promote cell attachment and expansion on the culture matrix and enhance the interaction between cells and the matrix.

Components

Instructions for Use

1. Stock solution Preparation.

One kit is for 50ml cell culture medium.

The compounds are offered as powder in tubes. Please centrifuge before opening the cap to ensure the accuracy of the dosage.

Please carry out dissolution and packaging operations on a clean bench.

Spray the medium bottle and supplement tube with 70% ethanol and wipe to remove excess liquid. In a sterile field, remove the caps without touching the interior  threads  with  fingers. 

Reconstitute NaC20120115-A in sterile 0.9% NaCl solution. Aliquot into appropriate volumes of storage solution. When stored at -20℃, the stock solution is stable for 6 month. When stored at 4 ℃, the stock solution is stable for 1 week.

Reconstitute NaC20120115-B in sterile 0.9% NaCl solution.  Aliquot into appropriate volumes of storage solution. When stored at 2-8 ℃, -20℃, the stock solution is stable for 24 month.

2. Reference Protocol

Step 1: Culture medium Preparation

a.  Thaw stock solutions of Lolina? Human UC-MSC stem-Prom? supplement kit 2.

b.  Dilute the stock solutions into regular culture medium without L-Glutamine to get complete culture medium.  

Step 2: Cell culture

c.   Rapidly thaw frozen UC-MSC vials in a 37°C water bath.

d.  Transfer cells to a sterile conical tube with pre-warmed medium.

e.   Centrifuge at 300g for 5 minutes to pellet cells.

f.   Resuspend the cell pellet in complete culture medium plate into culture dishes/flasks.

g.   Change the medium every 2-3 days.

Note

Cell Density: Avoid over-confluence to maintain MSC multipotency.

Medium Preparation: Always prepare fresh medium, even though SafeGluta? enhances stability.