詳細(xì)說明
Lolina A/SAddress: Sindalsvej 30 8240 Risskov Danmark
Email: Info@lolina.dk
Product Information
Product name | Lolina? L2 TaqMan Multiplex qPCR Probe Kit |
Cat.No. | NaM602008 |
Size | 100T/1000T /10000T |
Storage and shipping | 1. Shipping and Storage Instructions: The product is shipped with ice packs. 2. Store at -20°C. The product is stable for 24 months. |
Application equipment | Applied Biosystems: 5700, 7000, 7300, 7700, 7900HT Fast, StepOne?, StepOne Plus?, 7500, 7500 Fast, ViiA?7, QuantStudio? 3 and 5, QuantStudio? 6,7, 12k Flex; Bio-Rad: CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4; Eppendorf: Mastercycler ep realplex, realplex 2 s; Qiagen: Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000; Roche Applied Science: LightCycler 480, LightCycler 2.0; Lightcycler 96; Stratagene: MX3000P?, MX3005P?, MX4000P? ; Thermo Scientific: PikoReal Cycler; Cepheid: SmartCycler; Illumina: Eco qPCR. |
Product description
Lolina? L2 TaqMan Multiplex qPCR Probe Kit is a fluorescence-based quantitative PCR (qPCR) reagent that utilizes the company's next-generation antibody-mediated hot-start Taq enzyme. The 2× reaction buffer contains Mg2+ and dNTPs, along with factors that effectively inhibit non-specific PCR amplification and enhance the efficiency of multiplex qPCR reactions. This allows for up to fourplex reactions while ensuring primer efficiency.
Components
Components No. | Components Name | Size | ||
100T | 1000T | 10000T | ||
NaM602008-A | 2×TaqMan qPCR buffer | 1.25 mL | 12.5 mL | 125 mL |
NaM602008-B | Hotstart D -Taq（5 U/uL） | 60 μL | 600 μL | 6 mL |
Operate
qPCR reaction System
Components | Volume μL | Final Conc. |
2× TaqMan qPCR buffer | 12.5 | 1 × |
Hotstart D-Taq（5 U/μL） | 0.6 | 0. 12 U/μL |
Primer mix (10 μM)) | x | 0. 1 μM-0.5 μM |
Probe mix (10 μM) | x | 50 nM-250 nM |
DNA/cDNA template | 1-10 | - |
ddH2O | Up to 25 | - |
[Note]: Primer concentration and DNA amount can be adjusted according to experimental requirements.
Reaction ro ram
Cycle step | Temp. | Time | Cycles |
Initial denaturation | 95 °C | 5 min | 1 |
Denaturation | 95 °C | 15 sec | 45 |
Annealing/Extension | 60 °C | 30 sec |
[Note]: Annealing/extension temperature can be adjusted according to experimental requirements.
Notes
1. For your safety and health, please wear lab coats and disposable gloves for operation.
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