詳細(xì)說明
Lolina A/SAddress: Sindalsvej 30 8240 Risskov Danmark
Email: Info@lolina.dk
Website: https://lolina.dk
Product Specification
Product | Lolina? Hotstart Direct Taq DNA Polymerase, 5 U/μL |
Catalog No. | NaM203003 |
Storage conditions | Shipment with ice packs. Store at -20°C with a shelf life of 2 years. |
Hot Start | Built-in hot start |
Product description
Lolina? Hotstart Direct Taq DNA Polymerase is a heat-activated DNA polymerase that is tolerant to blood and other inhibitors. This product utilizes antibody blocking and possesses excellent amplification sensitivity and specificity. The antibody is fully deactivated by heating at the denaturation temperature for 30 seconds, releasing the DNA polymerase activity. The use of this hot- start Taq enzyme effectively suppresses non-specific amplification caused by primer misannealing.
Components
Catalog No. | Specification | Volume | |
Lolina? Hotstart Direct Taq DNA Polymerase, 5 U/μL | NaM203003ES72 | 250 U | 50 μL |
NaM203003ES76 | 500 U | 100 μL | |
NaM203003ES80 | 1000 U | 200 μL | |
NaM203003ES92 | 10 KU | 1 mL× 2 | |
NaM203003ES93 | 25 KU | 1 mL× 5 | |
NaM203003ES94 | 50 KU | 10 mL |
Instructions
1.Reaction System
Components | Volume（μL） | Final Concentration |
2×Buffera | 25 | 1 x |
Primer/Probe mixb | x | 0. 1 μM-0.5 μM |
Hotstart D-Taq (5 U/μL)c | 1.2 | 0. 12 U/μL |
Template DNAd | x | 0.1-100 ng |
ddH2O | up to 50 | - |
[Note]:
a) According to the specific experimental application, it is necessary to prepare the corresponding reaction buffer.
b) The primer concentrations in the table are recommended concentrations. They can be adjusted according to the specific experimental conditions to achieve the optimal concentrations.
c) Adjust the amount of Taq enzyme according to the specific experimental application.
d) The DNA amount in the table are recommended concentrations. They can be adjusted according to the specific experimental conditions to achieve the optimal concentrations.
Cyclestep | Temp. | Time | Cycles |
Initial denaturation | 95°C | 5 min | 1 |
Denaturation | 95°C | 15 s | 45 |
Annealing/Extension | 60°Ca | 30 sb |
[Note]:
a) Amplification reaction: The amplification reaction temperature should be adjusted based on the Tm value of the designed primers.
b) Fluorescence signal collection: Different qPCR instruments require different fluorescence signal collection times. Please set the collection time according to the shortest time limit.
Notes
For your safety and health, wear lab coats and disposable gloves during operation.
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